Dr. Vishakha Raina
Associate Professor, Associate Dean (Academic)
Ph. D. University of Delhi; 2004
M. Sc. University of Delhi; 1999


Email : vraina@kiitbiotech.ac.in

Postdoctoral Experience

Did postdoctoral research at Swiss Federal Institute of Aquatic Science and Technology (EAWAG), Dubendorf, Switzerland from 2005-2007 under the Indo-Swiss collaboration in Biotechnology (ISCB) jointly sponsored by DBT, GOI and SDC, Switzerland on "Bioremediation of HCH- and PNP-contaminated soils by bioaugumentation: Proof of concept at pilot scale".

Research Interest

  1. Bioprospecting and Metagenomics of India’s largest estuarine and brackish water Chilika lagoon for isolation of industrially important alkaliphilic and halophillic microbial enzymes (chitinase, protease, cellulase, lipase, pectinase, xylanase, amylase) and bioactive compounds (antimicrobial) beneficial for industry and humankind. This includes culture dependent and culture independent approaches to understand the role of micro-organisms and how they are involved in circulation of nutrients and maintenance of several biogeochemical cycles responsible for maintenance of biodiversity and ecological balance.
  2. Microbial biodegradation and bioremediation of Xenobiotic compounds especially organochlorines (Hexachlorocyclohexane, endosulfan) and organophosphates (chloropyrifos) from contaminated soil and wetlands.
  3. Biocontrol of (mosquito larvae) vectors causing Malaria, Dengue, Chikungunya using indigenous microbial strains of Bacillus thuringiensis.
  4. Waste Management of cooked food waste by using effective microorganism consortia from Chilika Lake and other environmental sources.
  5. Production of biodiesel and bioethanol from waste cooked vegetable oil and cooked food waste.

Selected Publications

Full List
  1. Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V. Streptomyces barkulensis sp. nov., isolated from estuarine Chilika Lake sediment in Odisha. Int J Syst Evol Microbiol. 2014, 64, 1365–1372
  2. Ray L, Suar M, Pattnaik A.K, Raina V. Streptomyces chilikensis sp. nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha. Int J Syst Evol Microbiol. 2013, 63, 2757–2764.
  3. Lal R, Pandey G, Sharma P, Kumari K, Malhotra S, Pandey R, Raina V, Kohler HP, Holliger C, Jackson C, Oakeshott JG. Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiol Mol Biol Rev. 2010, 74(1): 58-80.
  4. Raina, V, D. Rentsch, T. Geiger, S. Huber, P. Sharma, H.-R. Buser, C. Holliger, R. Lal, and H.-P.Kohler. New metabolites in the degradation of α- and γ-hexachlorocyclohexane (HCH): Pentachlorocyclohexenes are hydroxylated to cyclohexenols and cyclohexenediols by the haloalkane dehalogenase LinB from Sphingobium indicum B90A. 2008. J. Agric. Food Chem., 13; 56 (15): 6594-603
  5. Raina V, Hauser A, Buser HR, Rentsch D, Sharma P, Lal R, Holliger C, Poiger JT, Müller MD, Kohler HP. Hydroxylated metabolites of beta- and delta-hexachlorocyclohexane: bacterial formation, stereochemical configuration, and occurrence in groundwater at a former production site. Environ Sci Technol. 2007 Jun 15; 41(12): 4292-8.
  1. Bioprospecting and Metagenomics of brackish water Chilika lagoon for isolation of industrially important enzymes and bioactive compounds beneficial for humankind.

    The project “Isolation and characterization of benthic as well as littoral micro flora of Chilika Lake” covers a very important and popular biodiversity hotspot where several dynamic abiotic and biotic factor interplay for sustenance of biological life. Our aim in this project is to work upon methods which involve culture-based identification and non culture based identification and characterization of micro flora from Chilika Lake. These micro-organisms are important since they are involved in circulating nutrients and contribute to several biogeochemical cycles which are responsible for maintenance of the biosphere. These micro-organisms are being identified and screened for their biotechnological potential which can be used for potential bioprocesses which will help in creating  sustainable life in an around this ecosystem. Enzymes are the crux of biological product formation and are essential catalysts for carrying out reactions which yield biotechnologically and commercially important products. These enzymes can be isolated from microbes and used in industrial reactions.

    We use culture dependent isolation, screening (primary, secondary) of microbes for specific enzymes, identification (using phenotypic and genotypic, morphological, biochemical and molecular methods) and validation of biotechnological potential for bioprocesses.

    We have more than 500 isolates, which have been isolated from different sampling points throughout the lake across three major seasons i.e. summer, winter and rainy. These isolates have been screened for industrially important enzymes (lipases, amylases, proteases, chitinases, pectinases, cellulases and xylanses).  Screening for microbes producing enzymes is tedious process as the process is laborious and requires rigorous screening for selecting the right candidate for industrial operations. Hence, the process involves several level of primary, secondary and tertiary screening which ensures that the chosen microbe is commercially viable.

    After having conducted primary and secondary screening of isolates they are subject to other selectives pressures like tolerance to pH and salinity, since Chilika Lake is a brackish water wetland ecosystem and a wide range of such parameters ensures presence of extremophiles which can tolerate these conditions. This level of screening is being conducted at present for current isolates.  The identification of bacterial isolates is performed by 16S rRNA sequencing and data for selective strains is presented. As more and more isolates will be screened the identification will be conducted for them also.

    Besides isolation, the cultures are being purified as pure cultures after morphological identification and primary level morphological and physiological screening is conducted. After this cultures are preserved as pure culture stocks by cryopreservation and later will be freeze dried. For this an inventory of isolated strains is being prepared for different isolates.

    Water is an essential solvent medium for several nutrients and for exchange of gases. This medium is necessary for all biological systems and hence its quality needs to be monitored and assessed for health of biological communities. An important indicator of water quality is presence of FIB (faecal indicator bacteria). These FIB are common in faecal matter, are gram negative and are responsible for several; enteric diseases in fishes, birds and other brackish water animals. However, their number is kept in check by presence of marine viruses. We are conducting monitoring of FIB at different sampling points where there are more anthropogenic influences and presence of migratory birds (mainly central, south and north zones). The data will be compiled and presented in annual report.

    For culture independent approaches, we have isolated metagenomic DNA (total DNA of all organisms in an environmental sample) which is going to be sequenced by pyrosequencing (Roche 454-FLX, USA). This will help us to obtain detailed microbial data on all types and groups of microbial floral and communities existing in Chilika Lake.

  2. Biodegradation and bioremediation of Xenobiotic compounds especially organochlorines (Hexachlorocyclohexane) and organophosphates (Chlorpyrifos) from contaminated soil.

    In India, insecticides constitute the largest group (80%) of xenobiotics, among which chlorpyrifos (CP) has become the largest organophosphate (OP) insecticide in both volume and value. The sales of chlorpyrifos ranked the first among all OP pesticides worldwide, and the third among all pesticides. It is predicted that chlorpyrifos technical production in China and India will account for over 80% of the global total supply by 2015 i.e. more than 100,000 tonnes (World Outlook of Chlorpyrifos 2010-2015, CCM International). This means that intensification in use of CP will most likely increase the risk of environmental contamination and health hazards to consumers by residues accumulated on agri-crops.

    The major CP degradation product, TCP (3,5,6-trichloro-2-pyridinol) is more soluble, bioavailable and mobile, has antimicrobial activity and hence prevents growth of CP degrading micro-organisms. It has also been reported that efficient CP degradation best occurs co-metabolically because individual strains are not capable of removing TCP. Degradation of CP in acidic soils is slow but rate of degradation and hydrolysis is greatly increased by soil bacterial community on increase in soil pH and on repeated CP application. This increases prospects for developing efficient bio-stimulation and bio-augmentation potential for biodegradation of CP. Considering all this information studies on optimization and development of CP degrading technologies have not been intensively worked out.

    We are focussing on several new CP degrading isolates that have been isolated earlier from rice field soil following repeated application of chlorpyrifos are to be tested for CP degradation and verified, validated, identified and then optimized for developing new bioremediation technologies. CP is widely used in rice paddies (constructed secondary wetlands) where through run off CP can reach primary wetlands like lakes and other water bodies affecting ecological balance. Although several microbes are known to degrade CP as sole source of C, N or P (Xu et al., 2008; Li et al., 2011), mixed cultures have been shown to be more effective (Mallick et al., 1999; Guha et al., 1997).

    Hence we are currenly working on optimization of consortia capable of CP degradation in field and their efficiency (for degradation) in soil and water, interaction with other soil bacteria (exchange of plasmids for CP degradation) which need to be studied more intensively for developing an efficient bioremediation process. We also are standardizing effective carrier material (s) for immobilization of consortia and to ensure survivability and microbial CP degradation activity. In addtion, screening and validation of CP degrading microbes for presence of organophosphate degrading genes (oph, opd, mpd genes) as markers and community analysis in constructed secondary wetlands for identification and tracking of CP degrading microbes is also being conducted.

On going Research Projects:

Sl No. Title of Project Funding Agency Total Approved Cost of the Project
(In Rs.)
Date of sanction
and Duration
1. Isolation and Characterization of Benthic as well as Littoral Microbial Flora of Chilika Lake sponsored by World bank for three years (July, 2013). World Bank Rs. 1,78,00,000.00 3rd July, 2013
(Three years)
2. Development and Optimization of Technology for Bioremediation of Chlorpyrifos in Constructed Wetlands sponsored by DBT for three years (August, 2013). DBT, GOI Rs. 30,70,000.00 29th August 2013
(Three years)

Completed Research Projects

Sl No. Title of Project Funding Agency Total Approved Cost
of the Project (In Rs.)
Date of sanction
and Duration
1. Project entitled, “Environmental Risk and Bioremediation of Hexachlorocyclohexane Isomers”.
BT/IC-2/SWISS/LAL-RAINA/P3/2008-BN
Indo-Swiss Collaboration in Biotechnology (ISCB): Jointly sponsored by DBT, GOI and SDC, Switzerland. 5,54,100.00 17.11.2008- 31.08.2011;
Two years 8 months
 

Collaborators

  1. Chilika Development Authority (CDA), Chandraput, Odisha
  2. Prof. Timothy Vogel, University of Lyon, France: collaboration on sequencing and metagenomic analysis to study biodiversity of Chilika Lake, Odisha.
  3. Prof. Torsten Vennemann, University of Lausanne, Switzerland.
  4. Indo-Swiss Collaboration in Biotechnology (ISCB) project jointly sponsored by DBT, GOI and SDC, Switzerland with collaborators Prof. Christof Holliger (EPFL, Lausanne, Switzerland) and Dr. Hans Peter E. Kohler (EAWAG, Dubendorf, Switzerland)

Dr. Lopamudra Ray
Research Scientist
Email: loparay@gmail.com
Project : Chilika Project
Publications

  • Lopamudra Ray, Mrutyunjay Suar, Ajit Kumar Pattnaik, Vishakha Raina. Streptomyces chilikensis sp. nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha  International Journal of Systematic and Evolutionary Microbiology (2013), 63, 2757–2764
  • Lopamudra Ray, Samir Ranjan MishraAnanta Narayan Panda, Gurdeep Rastogi, Ajit Kumar Pattanaik, Tapan Kumar Adhya, Mrutyunjay Suar, Vishakha Raina. Streptomyces barkulensis sp. nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha  International Journal of Systematic and Evolutionary Microbiology (2014), 64, 1365–1372

Hobbies: Listening music, reading novels

Conference Attended:

  • Oral Presentataion:
    Ramakrishnan B.,Sinha S., Ray Lopamudra ., Samantray R.N., Mishra A.K., Rao V.R. (2004). Temperature dependence of methane production in anoxic rice soil under longterm monocropping management (Oral presentation) pp 25-26: In Indian Environment Congress 2004, Thiruvanantpuram

Posters presented in Conferences:

  • Ramakrishnan B., Ray Lopamudra.,Samantray R.N.,Mishra A.K.,Rao V.R(2004). Methanogenic potential of anoxic rice soil under long term nutrient management in tropical India (Abstract selected for poster presentataion in World Rice Research conference, Tokyo, November 4-7, 2004.
  • Ramakrishnan B., Prusty R., Ray Lopamudra., Samantray R.N., Mishra A.K., Rao V.R.(2005). Structural composition of microbial communities in anoxic rice soil under intensive rice cultivation of tropical India .In: Microbial Diversity 2005. International Conference-Microbial Diversity: Current perspective and potential application. April 16-18, 2005, New Delhi.
  • Lopamudra Ray, R.M Rathore, P. Luethy, M.Suar, J.S.Purohit ,Vishakha Raina. A Novel Producer of Chitinase from a unique habitat of Orissa : Applications and Prospective. AMI Conference 2008
  • Lopamudra Ray, R.M Rathore, P. Luethy, M.Suar, J.S.Purohit ,Vishakha Raina A study of chitinolytic actinomycete from chilika lake of orissa, india AMI Conference 2009
  • Lopamudra Ray, Thomas Poiger, Vishakha Raina Residues of Hexachlorocyclohexane isomers and metabolites in the agricultural and Industrial soil and water samples of Orissa
  • Lopamudra Ray, Purusottam Mohapatra, RanjanPreet, Chanakyanath Kundu,Vishakha Raina. Residues of Hexachlorocyclohexane isomers and metabolites in the agricultural / Industrial soil and water samples of Orissa and evaluation of the toxicity of these compounds in Human Breast Cancer Cell line.
  • Lopamudra Ray, Swatishree Padhi, Thomas Poiger, Birendranath Banerjee, Vishakha Raina. Residues of Hexachlorocyclohexane isomers and their degradation metabolites in the agricultural and Industrial soil samples of Odisha and the Evaluation of genotoxicty of Beta-HCH and its degradation metabolite by micronuclei assay.
  • Sameer R. Mishra, Anant N.Panda, Aditya Dash, Timothy M. Vogel, Lopamudra Ray, Luna Goswami, Vishakha Raina,Ajit k. Pattnaik, Mrutyunjay Suar. Culturable and Metagenomic approaches to find novel Bioactive compounds from Chilika Lake: The Future bioprospecting destination .
  • Lopamudra Ray , R.M.Rathore, Sameer R. Mishra, Sabyasachi Dash, Ananta N. Panda, Aditya Dash, Vishakha Raina, Luna Goswami, Archana Ghatak, Ajit K. Pattanaik, Mrutyunjay Suar. An investigation of novel multi-enzyme producing microorganisms from Chilika Lake: Study of a unique chitinase producing Streptomyces.

Samir Ranjan Mishra
Research Scientist
Email: mishra.samirr@gmail.com

Membership of Professional Associations:

  1. Life member of the Association of Microbiologists of India (AMI), New Delhi, India.
  2. Member of the American Society for Microbiology (ASM), Washington, D.C., USA.

Recent Publication:

  1. Arindam Giri, Tridib Bhunia, Samir Ranjan Mishra, Luna Goswami, Asit Baran Panda, Sagar Pal and Abhijit Bandyopadhyay (2013). Acrylic acid grafted guargum-nanosilica membranes for transdermal diclofenac delivery. Carbohydrate Polymers, 91:492-501.
  2. Lopamudra Ray, Samir Ranjan Mishra, Ananta Narayan Panda, Gurdeep Rastogi, Tapan Kumar Adhya, Ajit Kumar Pattanaik, Mrutyunjay Suar, Vishakha Raina(2014). Streptomyces barkulensis sp. nov., a halophilic streptomycete from estuarine Chilika Lake sediment in Odisha, International Journal of Systematic and Evolutionary Microbiology, 64, 1365–1372.
  3. Arindam Giri, Tridib Bhunia, Samir Ranjan Mishra, Luna Goswami, Asit B. Panda and Abhijit Bandyopadhyay (2014). A transdermal device from 2-hydroxyethyl methacrylate grafted carboxymethyl guar gum–multi-walled carbon nanotube composites. Royal Society of Chemistry. RSC Adv., 2014, 4, 13546.

Name: Anant Narayan panda
PhD Student
Email: anpandabiotek@gmail.com
Project: Chilika Project
Thesis Title: Study of novel proteases and Metagenomics of Chilika lake

Publications

Hobbies: Listening Music, Playing Cricket

Conference Attended

  • 53rd  Annual conference of Association of Microbiologist of India (AMI)”,
  • School of Biotechnology, KIIT University, India, 23rd – 25th November, 2013 [poster Presentation]

Name: M. Padma
PhD Student (Junior Research Fellow)
Email: padmabiotech22@gmail.com
Project: Chilika Project

Hobbies: reading books, playing carom

Conference Attended:

  • National conference Attended on “Peaceful Applications Of Atomic  Energy.” (February 22-23,2012) Organized By GITAM Institute Of Science & Public Awareness Division,Department Of Atomic Energy, Govt. Of India, Mumbai during 22-23 February, 2012 at GITAM University, Visakhapatnam.

Name: Tanmaya Nayak
PhD Student (Senior Research Fellow)
Email: tanmay.nayak07@yahoo.com

Project  and Thesis: “Development and Optimization of Technology for Bioremediation of Chlorpyrifos in Constructed Wetlands”

Publications:

  • Sanjukta Subudhi, Tanmaya Nayak, N. Ram Kumar, P. Vijaykanth, & Banwari Lal* (2013) Impact of regulated pH on proto scale hydrogen production from xylose by an alkaline tolerant novel bacterial strain, Enterobacter cloacae DT-1 (International Journal of Hydrogen Energy, 38;6:2728-2737)
    Hobbies: Photography, Playing cricket and badminton

Conference Attended:

  • 53rd  Annual conference of Association of Microbiologist of India (AMI)”,
  • School of Biotechnology, KIIT University, India, 23rd – 25th November, 2013 [poster Presentation]
  • International conference on Advances in Biological Hydrogen Production and Applications, JNTUH, Hyderabad, India, 14-15th December, 2012 [Oral Presentation]
  • 52nd Annual conference of Association of Microbiologist of India (AMI)” Punjab University, Chandigarh, India, 3-6th November, 2011 [poster Presentation]
  • 1st International Conference on Biogas Microbiology, Helmholtz Centre for Environmental Research – UFZ, 14th – 16th September, 2011 [poster Presentation]
  • International conference on NEW HORIZONS IN BIOTECHNOLOGY”. 21-24th November, 2011, Trivandrum, Kerala, India. [Poster Presentation]

Publication

  1. Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V. Streptomyces barkulensis sp. nov., isolated from estuarine Chilika Lake sediment in Odisha. Int J Syst Evol Microbiol. (2014), 64, 1365–1372
  2. Ray L, Suar M, Pattnaik A.K, Raina V. Streptomyces chilikensis sp. nov., a novel halophilic streptomycete isolated from brackish water sediment of Lake Chilika in Odisha. Int J Syst Evol Microbiol. 2013, 63, 2757–2764.
  3. Rout, R., Raina V, Suar M and Luethy,P. High prevalence of bacterial spore formers active against mosquito larvae in temporary monsoon flooded sites in Orissa, India. 2011. J. American Mosquito Control Association. 27(2): 159-161.
  4. Lal R, Pandey G, Sharma P, Kumari K, Malhotra S, Pandey R, Raina V, Kohler HP, Holliger C, Jackson C, Oakeshott JG. Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiol Mol Biol Rev. 2010, 74(1): 58-80.
  5. Raina, V, D. Rentsch, T. Geiger, S. Huber, P. Sharma, H.-R. Buser, C. Holliger, R. Lal, and H.-P.Kohler. New metabolites in the degradation of α- and γ-hexachlorocyclohexane (HCH): Pentachlorocyclohexenes are hydroxylated to cyclohexenols and cyclohexenediols by the haloalkane dehalogenase LinB from Sphingobium indicum B90A. 2008. J. Agric. Food Chem., 13; 56 (15): 6594-603
  6. Raina V, Suar M, Singh A, Prakash O, Dadhwal M, Gupta SK, Dogra C, Lawlor K, Lal S, van der Meer JR, Holliger C, Lal R. Enhanced biodegradation of hexachlorocyclohexane (HCH) in contaminated soils via inoculation with Sphingobium indicum B90A. Biodegradation. 2008 Feb; 19(1): 27-40.
  7. Raina V, Hauser A, Buser HR, Rentsch D, Sharma P, Lal R, Holliger C, Poiger JT, Müller MD, Kohler HP. Hydroxylated metabolites of beta- and delta-hexachlorocyclohexane: bacterial formation, stereochemical configuration, and occurrence in groundwater at a former production site. Environ Sci Technol. 2007 Jun 15; 41(12): 4292-8.
  8. Sharma P, Raina V, Kumari R, Malhotra S, Dogra C, Kumari H, Kohler HP, Buser HR, Holliger C, Lal R. Haloalkane dehalogenase LinB is responsible for beta- and delta-hexachlorocyclohexane transformation in Sphingobium indicum B90A. Appl Environ Microbiol. 2006 Sep; 72(9): 5720-7.
  9. Suar, M., A. Hauser, T. Poiger, H. R. Buser, M. D. Muller, C. Dogra, V. Raina, C. Holliger, J. R. van der Meer, R. Lal, and H. P. Kohler. 2005. Enantioselective transformation of a-Hexacholorocyclohexane by the dehydrochlorinases LinA1 and LinA2 from the soil bacterium Sphingomonas paucimobilis B90A. Appl. Environ.  Microbio. 71:8514-18.
  10. Prakash O., M. Suar, V. Raina, C. Dogra, R Pal, and R. Lal. 2004. Residues of hexacholorocyclohexane isomers in soils and water samples from Delhi and adjoining areas. Current Science. 87:73-77.
  11. Dogra, C., V. Raina, R. Pal, M. Suar, S. Lal, K. –H Gartemann, J. R. van der Meer, C. Holliger, and R. Lal. 2004. Organization of lin Genes and IS6100 among different strains of Hexachlorocyclohexane degrading Sphingomonas paucimobilis - Evidence for horizontal gene transfer.  J. Bacteriol. 186:2225-35.
  12. Kumari, R., S. Subudhi, M. Suar, G. Dhingra, V. Raina, C. Dogra, S. Lal, J. R. van der Meer, C. Holliger, and R. Lal. 2002. Cloning and chracterization of lin genes responsible for the degradation of Hexachlorocyclohexane isomers by Sphingomonas paucimobilis B90. Appl. Environ. Microbiol. 68:6021-28.